Journal: Molecular Neurodegeneration

Article Title: Intracellular amyloid formation in muscle cells of Aβ-transgenic Caenorhabditis elegans : determinants and physiological role in copper detoxification

doi: 10.1186/1750-1326-4-2

Figure Lengend Snippet: Transgenic C. elegans strains express the Aβ wild type and its variants Arctic and NIC . A . Amino acidic sequences of the Aβ 1–42 peptide (wild type) and its variants Arctic and NIC expressed in the transgenic C. elegans strains used in this study. B . PCR results show the presence of the DNAs that encode the different peptides in the transgenic strains. CL2120 is the strain that express Aβ wt and CL2122 is the control strain that do not express the peptide. NIC corresponds to transgenic line NIC2 and Arctic corresponds to transgenic line Arctic 6. unc-119 is the strain used to construct the transgenic lines carrying the NIC and Arctic variants. C . Western blot analysis using the anti-Aβ antibody 6E10 showing the expression of Aβ in the CL2120 (wild type Aβ) strain and in two NIC transgenic lines (NIC 1 and NIC 2). The lane marked as "no Aβ " correspond to strain CL2122. D . Western blot analysis using the same antibody as in C showing the expression of Aβ in strain CL2120 (wild type Aβ) and in four Arctic transgenic lines (Arctic 13, Arctic 6, Arctic 37, Arctic 5D). The lane marked as "no Aβ " correspond to strain CL2122. α-Tubulin was used as loading control in C and D.

Article Snippet: We used the anti-Aβ monoclonal antibody 6E10 (Chemicon) at 1:1,000 dilution or anti-α-tubulin monoclonal antibody (Sigma) at 1:5,000 dilution overnight at 4°C.

Techniques: Transgenic Assay, Construct, Western Blot, Expressing